WebJun 1, 2024 · Real-space refinement in PHENIX for cryo-EM and crystallography. This article describes the implementation of real-space refinement in the phenix.real_space_refine … WebPhenix A comprehensive software package for macromolecular structure determination using crystallographic (X-ray, neutron and electron) and electron cryo-microscopy data. Learn more NEW: Phenix with AlphaFold models Trim, weight, create domains and use for molecular replacement Trim, dock into cryo-EM maps and fill in gaps
phenix.real_space_refine: a tool for refinement a model against a …
WebSep 22, 2024 · Samples of purified virus prepared as described above (in PBS containing approximately 30% sucrose) were adjusted to pH 6.3 by 10-fold dilution in 107 mM NaCl, 50 mM citric acid:sodium phosphate, 0.1mg/ml BSA, 2mM CaCl 2 pH 6.3 for 2 minutes at room temperature to promote capsid dissociation, then neutralised by addition of HEPES pH 8.0 … WebJan 17, 2024 · This article describes& the& implementation& of& realNspace& refinement& in& the& phenix.real_space_refine& program from& the& Phenix& suite.& Use& of a simplified& refinement& targetfunctionenables&fast calculation,&which&inturn makes&it&possible&to&identify&optimal& take a walk with me lyrics
Phenix User Workshop 9/16/2024 - phenix.real_space_refine
WebDec 6, 2024 · Hello, I am trying to use phenix.real_space_refine to improve the fit between my protein's ligand and the electron density. I include a CIF file that has worked well for reciprocal space refinement, made through JLigand. It seems to me that the CIF file is read properly, it does not produce errors and the restrains are stated correctly in the ... WebJun 1, 2024 · Synopsis A description of the implementation of real-space refinement in the phenix.real_space_refine program from the Phenix suite and its application to re … WebJun 1, 2024 · This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified … take aware of it meaning