Lineage tracing studies
Nettet19. apr. 2024 · A recent study integrating lineage tracing and spatial transcriptomics has demonstrated that the closely located cells tend to share lineage origins in cerebral organoids (He et al., 2024). This phenomenon may not be shared by the development of neural crest cells and some other long-traveling cells, as their derivatives are spread … Nettet8. mai 2024 · Here, we review the history of cell-tracing strategies and then elaborate on the working principle and application of a recently developed dual genetic lineage-tracing approach for cell fate studies. We place an emphasis on discussing the technical strengths and caveats of different methods, with the goal to develop more specific and …
Lineage tracing studies
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Nettet20. feb. 2024 · Our study provides a cellular landscape of CD34 + cell-derived cells in the hypertrophy heart of human and animal models, indicating ... By combining genetic lineage tracing and bone marrow transplantation models, we demonstrated that non-bone-marrow-derived CD34 + cells give rise to fibroblasts and endothelial cells, ... Nettet1. sep. 2024 · Lineage tracing is a powerful method to delineate all progenies produced by a single cell, which can be achieved by site-specific recombinase (SSR) technology. SSR technology was pioneered in the 1980s [ 4 ].
Nettet5. sep. 2024 · Single-cell lineage tracing permits the labeling of individual cells with a heritable marker to follow the fate of each cell’s progeny. Over the last twenty years, several single-cell lineage tracing methods have emerged, enabling major discoveries in developmental biology, oncology and gene therapies. Analytical tools are needed to … Nettet24. jun. 2024 · Similar lineage-tracing studies have also been performed in breast, prostate, and lung cancer models. Quinn et al. traced metastatic dissemination of xenograft A549 lung cancer cells and revealed different seeding topologies, including self-seeding and cascade seeding (Quinn et al., 2024. Quinn J.J.
Nettet1. jan. 2024 · Genetic lineage-tracing has provided a powerful strategy to unveil the heterogeneous nature of stem cells and their descendants. However, recent studies have reported controversial data regarding the heterogeneity of neural stem cells in the developing mouse neocortex, which prevents a decisive conclusion on this issue. Nettet21. des. 2024 · It is a succinct review and does cover majority of the current lineage tracing studies, although there have been a few recent articles publish which should be incorporated [Rädler PD, Wagner KU. Sci Rep. 2024; Lee HJ, Jang B. Sci Rep. 2024; Ginzel JD, Snyder JC. Mol Cancer Res. 2024], as well as some these articles [Sikandar …
Nettet3. mar. 2024 · Other studies available in bioRxiv used similar lineage tracing strategies and found that depleting PTBP1 was not able to convert astrocytes to neurons in the midbrain or elsewhere 6, 7. These observations raise serious questions about what type of evidence is needed to rigorously demonstrate direct conversion or cell reprogramming.
Nettet24. jun. 2024 · applied a powerful lineage tracing system with scRNA-seq to a pancreatic cancer metastasis model and revealed predominating hybrid EMT states in rare metastatic subclones. Similar lineage-tracing studies have also been performed in breast, prostate, and lung cancer models. fr lawrence bock ctNettet20. jan. 2012 · Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. fr lawn serviceNettetTo study the cell dynamics of hematopoiesis, without perturbation in their native niche, researchers have developed mouse models with endogenous single-cell lineage tracing systems, which can simultaneously trace thousands of hematopoietic progenitor cells in a single mouse, without transplantation. fcvc chinaNettet12. mai 2015 · When a lineage tracing study identifies cells with the capacity to self-renew and differentiate, one can make a positive conclusion regarding the existence of a progenitor population. If the... fr lawNettetLineage tracing is the term for a set of methods that allow one to follow successive cell progenies with minimal disturbance of their physiological function. Broadly, these methods can be divided into two categories: prospective and retrospective ( Figure 1 ). fr larry riceNettet2. des. 2024 · Lineage tracing studies have supported a luminal cell of origin of neuroendocrine prostate cancer cells that arise upon potent androgen blockade, supporting a “transdifferentiation” process. Important questions concerning the timing, cooperation, and feedback of the drivers of transdifferentiation processes and their … fcvb section sportiveNettet23. jun. 2024 · Cell lineage tracing experiments are of primordial importance in the study of tissue development and regeneration. By allowing to follow one cell and its entire progeny, this classical approach in developmental biology has provided crucial insights on the developmental and regenerative potential of embryonic and adult stem/progenitor … fcvb red star